Medium optimization for the production of glucose isomerase from thermophilic Streptommsces thermonitrificans.
Protein measurement with folin- phenol reagent. Lowry, O.H., Rosebrough, N.J., Farr, A.L. Colorimetric estimation of ketopentoses and ketohexoses.
GALLENKAMP INCUBATOR MANUALS PLUS MANUAL
Bergey’s Manual of Determinative Bacteriology (9 th ed.) Baltimore: Williams and Wilkins. Holt, J.G., Krieg, N.R., Sneath, P.H.A., Staley J.T. Kinetics of a three – step isomerization of glucose to fructose using immobilized enzyme. Isoglucose production from raw strachy materials based on a two-stage enzymatic system. Consumption of high – fructose corn syrup in beverages may play a role in the epidemic of obesity. Purification and characterization of thermostable glucose isomerase from Clostridium thermosulfurogenes and Thermoanaerobacter strain B6A. The thermostable glucose isomerase from psychrotolerant Streptomyces species.
Dhungel, B., Subedi, M., Tiwari, K.B., Shrestha, U.T., Pokhrel, S.
Codon optimization of xylA gene for recombinant glucose isomerase production in Pichia pastoris and fed-batch feeding strategies to fine-tune bioreactor performance. Recombinant protein production by sucrose-utilizing Escherichia coli W: untreated beet molasses-based feeding strategy development. Beet molasses based exponential feeding strategy for thermostable glucose isomerase production by recombinant Escherichia coli BL21 (DE3). Production and optimal performance studies of glucose isomerase from agriculture raw material Global Adv. Lawal, A.K., Banjoko, A.M., Banjoko, A.M., Osikoyia, A.F., Olatope, S.O., Kayode, O.F., Etoamihe, M., Emoleila., I., Alebiosu, F.A., Majolagbe, Y.L, Shittu, K.A, Buhari, F. Glucose isomerisation in simulated moving bed reactor by glucose isomerase. Silva, E.A.B, Souza, A.A.U., Rodrigues, A.E. Application of a compatible xylose isomerase in simultaneous bioconversion of glucose and xylose to ethanol. Molecular and industrial aspects of glucose isomerase. The optimum pH for best enzyme activity was 6.0 while optimum temperature for enzyme production was 50✬.ġ. Best enzyme activity was observed in the presence of Mg 2+ (specific activity = 6.85 U/mg protein) while Hg 2+ was inhibitory (specific activity = 1.02 U/mg protein). In general, organic nitrogen substrates supported higher enzyme productivity than inorganic nitrogen substrates. Lowest enzyme activity was observed with sodium trioxonitrate (specific activity = 2.44 U/mg protein). The effects of nitrogen substrates on glucose isomerase production showed that yeast extract supported maximum enzyme activity (specific activity = 5.24 U/mg protein). Media containing only xylose or glucose gave lower enzyme productivies (specific activities= 4.60 and 2.35 U/mg protein respectively). Enzyme was produced using a variety of carbon substrates but the highest enzyme activity was detected in a medium containing 0.5% xylose and 1% glycerol (specific activity = 6.88 U/mg protein). The bacterium was selected based on the release of 3.62 mg/mL fructose from the fermentation of glucose. This work reports the effects of some culture conditions on the production of glucose isomerase by Bacillus licheniformis.
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